Pain, one produced by needle? (6)
I believe the answer is:
stitch
This clue is a double definition.
'pain' is the first definition.
(stitch is a kind of pain)
'one produced by needle?' is the second definition.
(Other definitions for stitch that I've seen before include "A result of running" , "Application of needle produces this" , "Tack; pain in side" , "closer" , "Pain in the side brought on by exercise" .)