Play with tot first of all (5)
I believe the answer is:
drama
'play' is the definition.
('drama' can be a synonym of 'play')
'with tot first of all' is the wordplay.
'with' says to put letters next to each other.
'tot' becomes 'dram' (I've seen this before).
'first of' indicates taking the first letters.
The initial letter of 'all' is 'a'.
'dram'+'a' is 'DRAMA'.
(Other definitions for drama that I've seen before include "sensation" , "Literary genre for the theatre" , "Highly emotional episode" , "Exciting play or event" , "theatre studies" .)