Possibly using medium of culture noire, short ITV broadcast (2,5)
I believe the answer is:
in vitro
'possibly using medium of culture' is the definition.
(in vitro can mean taking place in a culture dish)
'noire short itv broadcast' is the wordplay.
'short' means to remove the last letter.
'broadcast' indicates an anagram.
'noire' with its last letter taken off is 'noir'.
'noir'+'itv'='noiritv'
'noiritv' is an anagram of 'IN VITRO'.
(Other definitions for in vitro that I've seen before include "Made to occur outside body (of biological process)" , "Via a test tube" , "- - fertilisation (IVF)" , "Done in a test tube" , "Taking place in a test tube (Latin)" .)