Rose and Gail unsettled in harem (8)
I believe the answer is:
seraglio
'harem' is the definition.
('seraglio' can be a synonym of 'harem')
'rose and gail unsettled' is the wordplay.
'and' is a charade indicator (letters next to each other).
'unsettled' indicates an anagram.
'rose'+'gail'='rosegail'
'rosegail' anagrammed gives 'SERAGLIO'.
'in' acts as a link.
(Other definitions for seraglio that I've seen before include "Muslim wives" , "I've made a mistake!" , "Harem - eg sailor (anag)" , "Group of women" , "Harem - or silage (anag)" .)