Seemed ill at ease and got all mixed up (8)
I believe the answer is:
shuffled
'mixed up' is the definition.
(thesaurus)
'seemed ill at ease and got all' is the wordplay.
I cannot really understand how this works, but
'got' could be 'led' (leading is a kind of getting) and 'led' is present in the answer.
This may be the basis of the clue (or it may be nonsense).
Can you help me to learn more?
(Other definitions for shuffled that I've seen before include "Rearranged (a pack)" , "Mixed at random - shambled" , "Rearranged (cards)" .)