She tempts one again into wrongdoing (5)
I believe the answer is:
siren
'she tempts' is the definition.
(I've seen this before)
'one again into wrongdoing' is the wordplay.
'one again' becomes 're' (prefix meaning 'again').
'into' is an insertion indicator.
'wrongdoing' becomes 'sin' (sin is a kind of wrongdoing).
're' put inside 'sin' is 'SIREN'.
(Other definitions for siren that I've seen before include "Temptress - makes a loud noise!" , "Charmer" , "Attractive lady" , "'Delilah!'" , "Hooter - femme fatale" .)