Shoot southern puritan (5)
I believe the answer is:
sprig
'shoot' is the definition.
(I've seen this before)
'southern puritan' is the wordplay.
'southern' becomes 'S' (abbreviation).
'puritan' becomes 'prig' (I've seen this before).
's'+'prig'='SPRIG'
(Other definitions for sprig that I've seen before include "Young family member; small stem" , "Stem of holly, for example" , "A child" , "Small branch or shoot" , "Small twig" .)