Spanish queen, the first murder victim in one’s city? (8)
I believe the answer is:
isabella
'spanish queen' is the definition.
(I've seen this before)
'the first murder victim in one's city?' is the wordplay.
'the first murder victim' becomes 'abel' (I've seen this before).
'in' is an insertion indicator.
'one's' becomes 'I's' (Roman numeral).
'city?' becomes 'LA' (Los Angeles).
'is'+'la'='isla'
'abel' going inside 'isla' is 'ISABELLA'.
(Other definitions for isabella that I've seen before include "Spanish queen who was patron of Christopher Columbus" , "A queen of Spain" , "Wife of Edward II" , "Name of two Spanish queens" , "Woman" .)