Spartan queen Dale upset (4)
I believe the answer is:
leda
'spartan queen' is the definition.
(I've seen this in another clue)
'dale upset' is the wordplay.
'upset' indicates an anagram.
'dale' is an anagram of 'LEDA'.
(Other definitions for leda that I've seen before include "- - and the Swan (mythology)" , "Mother of Helen and Pollux" , "small satellite of Jupiter" , "One ravished" , "Queen of Sparta, mother of Helen of Troy" .)