Speed at sea in bark? No, trawler! (4)
I believe the answer is:
knot
'speed at sea' is the definition.
(measure of speed once used on ships)
'in bark? no trawler' is the wordplay.
'in' indicates the answer is hidden within the clue.
'KNOT' can be found hidden inside 'bark no trawler'.
(Other definitions for knot that I've seen before include "Nautical speed - group of toads" , "Eg, hitch" , "Hard round cross-grained piece in wood" , "Tangled mass" , "Fastening; sea bird" .)