Spirit used in experiment (4)
I believe the answer is:
peri
'spirit' is the definition.
(peri is a kind of spirit)
'in experiment' is the wordplay.
'in' says the answer is hidden in the clue.
'PERI' is hidden in the letters of 'experiment'.
'used' acts as a link.
(Other definitions for peri that I've seen before include "Beautiful fairy-like creature" , "Type of disembodied spirit or graceful young girl" , "Fairy could be ripe" , "Spirit of folklore" , "Fairy of Persian folklore" .)