Stain in muddy England (3)
I believe the answer is:
dye
'stain' is the definition.
(I know that stain is a kind of dye)
'in muddy england' is the wordplay.
'in' indicates a hidden word.
'DYE' is hidden in the letters of 'muddy england'.
(Other definitions for dye that I've seen before include "Staining material" , "Colorant" , "Soluble substance for staining or colouring" , "Hair colouring" , "Colouring agent" .)