Stake in giant enterprise (4)
I believe the answer is:
ante
'stake' is the definition.
(I've seen this before)
'in giant enterprise' is the wordplay.
'in' indicates the answer is hidden within the clue.
'ANTE' can be found hidden inside 'giant enterprise'.
(Other definitions for ante that I've seen before include "Preposition meaning before" , "Poker pot starter" , "Betting stake" , "advance payment" , "'Behind, before (4)'" .)