The devil in Desdemona (5)
I believe the answer is:
demon
'the devil' is the definition.
('demon' can be a synonym of 'devil')
'in desdemona' is the wordplay.
'in' indicates the answer is hidden within the clue.
'DEMON' can be found hidden inside 'desdemona'.
(Other definitions for demon that I've seen before include "Genius" , "malignant spirit" , "Skilful person" , "See 3" , "Familiar" .)