They are associated with Queens Regulations (5)

I believe the answer is:

rules

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'regulations' is the definition.
('rule' can be a synonym of 'regulation')

I don't understand the rest of the clue.

(Other definitions for rules that I've seen before include "Is king" , "Governs; regulations" , "following Queensberry?" , "Reigns" , "Regulations, precepts" .)

I've seen this clue in The Irish News.

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