Though jesting, he is in fact wild (5)
I believe the answer is:
joker
'though jesting he is in fact wild' is the definition.
The answer is a person as well as being a singular noun. This is suggested by the definition.
'though jesting he' is the wordplay.
'though jesting' becomes 'jo' (I can't explain this - if you can you should believe this answer much more).
'he' becomes 'Ker'.
'jo'+'ker'='JOKER'
Can you help me to learn more?
(Other definitions for joker that I've seen before include "may appear wild in play" , "trickster" , "Playing card" , "Comic; a card" , "Jester" .)