Ticklish 'indrance over penultimate letter (5)
I believe the answer is:
itchy
'ticklish' is the definition.
(thesaurus)
''indrance over penultimate letter' is the wordplay.
''indrance' becomes 'itch' ('hitch' - hitch is a kind of hindrance - said with the 'H' dropped, a cockney accent, like 'hindrance' was).
'over' is a charade indicator (letters next to each other).
'penultimate letter' becomes 'y'.
'itch'+'y'='ITCHY'
(Other definitions for itchy that I've seen before include "Suffering from a rash?" , "Causing irritation to skin" , "Wanting a scratch?" , "Unable to relax" , "Have . . . . . feet - be restless, want to travel" .)