Tries once more removing Bottom from what actors do with play (7)
I believe the answer is:
rehears
'tries once more' is the definition.
('hears' can be a synonym for 'tries' so perhaps 'rehears' is OK for 'tries once more')
'removing bottom from what actors do with play' is the wordplay.
'removing bottom from' means to remove the last letter.
'what actors do with play' becomes 'rehearse'.
'rehearse' with its final letter removed is 'REHEARS'.
(Other definitions for rehears that I've seen before include "judges over" , "Once again tries" .)