Two fools at home hit man
I believe the answer is:
assassin
'hit man' is the definition.
(I've seen this in another clue)
'two fools at home' is the wordplay.
'two fools' means two substitutions for 'fool'.
'fool' becomes 'ass' (both can mean a stupid person).
'fool' becomes 'ass' (both can mean a stupid person).
'at home' becomes 'in' ('I'm in' can mean 'I'm at home').
'ass'+'ass'+'in'='ASSASSIN'
(Other definitions for assassin that I've seen before include "One murders for political or monetary reasons" , "Hired murderer" , "Killer of famous person" , "Killer of prominent person" , "Political/religious killer" .)