Villain accent initially lacking (5)
I believe the answer is:
rogue
'villain' is the definition.
(I know that rogue is a type of villain)
'accent initially lacking' is the wordplay.
'accent' becomes 'brogue' (brogue can mean a Scottish or Irish accent).
'initially lacking' means to remove the first letter.
'brogue' with its initial letter removed is 'ROGUE'.
(Other definitions for rogue that I've seen before include "Bad guy" , "Baddie" , "Unprincipled man" , "Baddy" , "Fraud" .)