Virginia upset young female subordinate (6)
I believe the answer is:
vassal
'subordinate' is the definition.
(I've seen this in another clue)
'virginia upset young female' is the wordplay.
'virginia' becomes 'va' (US state abbreviation).
'upset' shows that the letters should be reversed in order.
'young female' becomes 'lass'.
'lass' reversed gives 'ssal'.
'va'+'ssal'='VASSAL'
(Other definitions for vassal that I've seen before include "Medieval land-holder" , "One owed allegiance to a feudal lord" , "Feudal underling" , "Person or nation subject to another" , "One owing fealty" .)