Have last of cheeseboard on top of port (5)
I believe the answer is:
dover
'port' is the definition.
(English port)
'have last of cheeseboard on top of' is the wordplay.
'have last of' suggests the final letters.
'on top of' becomes 'over' (I've seen this in another clue).
The last letter of 'cheeseboard' is 'd'.
'd'+'over'='DOVER'
(Other definitions for dover that I've seen before include "The largest English ferry port" , "There'll be blue clouds over the white cliffs here" , "Be aware of" , "White cliffs port" , "UK shipping forecast area" .)