Left in the last queue (4)
I believe the answer is:
line
'queue' is the definition.
(a line of people)
'left in the last' is the wordplay.
'left' becomes 'l' (common abbreviation).
'last' indicates one should take the final letters.
The last letter of 'the' is 'e'.
'l'+'in'+'e'='LINE'
(Other definitions for line that I've seen before include "Wrinkle, boundary" , "Family" , "Row; track" , "Row - occupation" , "Winter sports slope" .)