A number of kittens (3)
I believe the answer is:
ten
'a number' is the definition.
(ten is a number)
'of kittens' is the wordplay.
'of' indicates the answer is hidden within the clue.
'TEN' is hidden in the letters of 'kittens'.
(Other definitions for ten that I've seen before include "How many" , "X-WORD" , "time" , "Base of decimal system" , "PM's address, Downing St" .)