For us to get Rosie upset is no laughing matter! (7)

I believe the answer is:
serious
'no laughing matter' is the definition.
(thesaurus)
'for us to get rosie upset' is the wordplay.
I cannot quite see how this works, but
'us' is present in the answer.
an anagram of 'rosie' is 'serio' which is located in the answer.
No letters remain.
This may be the basis of the clue (or it may be nonsense).
'is' is the link.
Can you help me to learn more?
(Other definitions for serious that I've seen before include "Earnest; vital" , "Stern" , "Not light or frivolous" , "Earnest - important" , "Earnest, sincere" .)
