Game at Twickenham English regret (3)
I believe the answer is:
rue
'regret' is the definition.
(to rue something is to regret it)
'game at twickenham english' is the wordplay.
'game at twickenham' becomes 'RU' (rugby union - Twickenham is a rugby stadium).
'english' becomes 'E' (abbreviation).
'ru'+'e'='RUE'
(Other definitions for rue that I've seen before include "Deplore" , "Repentant sort of shrub?" , "Feel sorry" , "Regret in a French street" , "Fish eggs" .)