Last hybrid, cat and horse (8)
I believe the answer is:
stallion
'horse' is the definition.
(I know that Stallion is a type of horse)
'last hybrid cat' is the wordplay.
'hybrid' indicates an anagram (a hybrid is a mixture).
'cat' becomes 'lion' (lion is a kind of cat).
'last' anagrammed gives 'stal'.
'stal'+'lion'='STALLION'
'and' acts as a link.
(Other definitions for stallion that I've seen before include "Stud" , "Male horse kept for breeding" , "Uncastrated adult male horse" , "Mare's mate" , "Mount" .)