Ned's up in his little room (3)
I believe the answer is:
den
'room' is the definition.
(I know that den is a type of room)
'ned's up in his little' is the wordplay.
'ned' can be anagrammed to 'DEN'.
But, I am not clear how the anagram is indicated.
Can you help me to learn more?
(Other definitions for den that I've seen before include "Animal's lair or man's study" , "Animal's home or gent's study" , "Lair for animal or study for gent" , "Home of the beast" , "Habitation of wild animals (3)" .)