Opposed to puncturing starch in the City (8)
I believe the answer is:
santiago
'the city' is the definition.
(Santiago is an example)
'opposed to puncturing starch' is the wordplay.
'opposed to' becomes 'anti' (I've seen this before).
'puncturing' is an insertion indicator (I've seen this in other clues).
'starch' becomes 'sago' (sago is a kind of starch).
'anti' going into 'sago' is 'SANTIAGO'.
'in' is the link.
(Other definitions for santiago that I've seen before include "City" , "Chile's capital" , "South American capital" , "Capital of Chile" , "Chilean capital" .)