Prank against Charlie (5)
I believe the answer is:
antic
'prank' is the definition.
(I know that prank can be written as antic)
'against charlie' is the wordplay.
'against' becomes 'anti' (prefix meaning against).
'charlie' becomes 'c' (phonetic alphabet: alpha, bravo, charlie etc.).
'anti'+'c'='ANTIC'
(Other definitions for antic that I've seen before include "Caper; grotesque (arch.)" , "Mad act done for fun" , "Playful or mischievous act" , "old buffoon" , "Eccentric once" .)