Run through gardens in series (6)
I believe the answer is:
skewer
'run through' is the definition.
(both can mean to impale)
'gardens in series' is the wordplay.
'gardens' becomes 'Kew' (Kew Gardens in London).
'in' means one lot of letters goes inside another.
'series' becomes 'ser' (abbreviation for series).
'kew' inserted within 'ser' is 'SKEWER'.
(Other definitions for skewer that I've seen before include "Displayer of meat" , "The heart of kebab" , "Spike on which kebab is cooked" , "part of barbecue" , "Pin used in cooking" .)