Transport gold in two hands by end of day (5)
I believe the answer is:
lorry
'transport' is the definition.
(lorry is a kind of transport)
'gold in two hands by end of day' is the wordplay.
'gold' becomes 'or' ('or' is the heraldic name for gold).
'in' means one lot of letters goes inside another.
'two hands' becomes 'lr' (abbreviation for left and right).
'by' is a charade indicator (letters next to each other).
'end of' indicates one should take the final letters.
The final letter of 'day' is 'y'.
'or' inserted into 'lr' is 'lorr'.
'lorr'+'y'='LORRY'
(Other definitions for lorry that I've seen before include "Large motor vehicle for transporting goods" , "From which stolen goods fall?" , "Load-carrying vehicle" , "HGV" , "Transport vehicle" .)