Unease of party male built up defence (10)
I believe the answer is:
discomfort
'unease' is the definition.
(I've seen this in another clue)
'party male built up defence' is the wordplay.
'party' becomes 'disco' (dance party).
'male' becomes 'm' (common abbreviation).
'built up defence' becomes 'fort' (I can't justify this - if you can you should believe this answer much more).
'disco'+'m'+'fort'='DISCOMFORT'
'of' is the link.
Can you help me to learn more?
(Other definitions for discomfort that I've seen before include "Unease" , "tenderness?" , "uneasiness" , "pain" , "Slight anxiety" .)