Unease of party male built up defence (10)
I believe the answer is:
discomfort
'unease' is the definition.
(I've seen this before)
'party male built up defence' is the wordplay.
'party' becomes 'disco' (dance party).
'male' becomes 'm' (common abbreviation).
'built up defence' becomes 'fort' (I can't justify this - if you can you should give a lot more credence to this answer).
'disco'+'m'+'fort'='DISCOMFORT'
'of' acts as a link.
Can you help me to learn more?
(Other definitions for discomfort that I've seen before include "Unease" , "tenderness?" , "uneasiness" , "pain" , "Slight anxiety" .)